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Fig. 4. Attraction of tangentially migrating cells to netrin 1. (A-D)
Co-cultures of rhodamine-labeled strips with aggregates (blue outline) of HEK
cells expressing netrin 1 (A,B) and the mock control cells (C,D). The boundary
between the neocortex and the GE is stained with anti-reticulon 1 antibody
(green in A,C). Many rhodamine-labeled cells migrate toward the netrin
1-secreting cells, but not the control cells. (E) The Dcc
mutant strip labeled with rhodamine and cultured for 2 days. The cells migrate
normally to the LOT area. (F) Co-culture of the Dcc mutant
strip with a cell aggregate expressing netrin 1 (blue outline). The cells are
not attracted to the aggregate. (G) The Dcc mutant strip
cultured with 10 µM cyclopamine. The migration of rhodamine-labeled cells
is not affected by the treatment. (H) The percentage of dye-labeled
cells that reside in the halves of strips proximal to cell aggregates. The
average values and the standard errors (thin horizontal bars) are calculated
from the wild-type strips cultured with mock-transfected cells (upper) and
netrin 1-expressing cells (middle) and Dcc homozygous mutant strips
culture with netrin 1-expressing cells (bottom). The numbers of strips used
for quantification are indicated by n in the parentheses. Scale bars: 500
µm.
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