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Figure 6


Fig. 6. Distribution of lot cells in the telencephalon of netrin 1 mutant embryos. Telencephalons of heterozygous (A,C,D,F,G,I,K) and homozygous (B,E,H,J,L) netrin 1 knock-in mutant embryos stained with mAb lot1 (A,B,D,E,G-L), anti-neuropilin 1 antibody (K,L) and X-gal solution (C,F,G-J). (A,B) At E12.5, distributions of lot cells in heterozygous and homozygous mutants are indistinguishable. (D,E) At E13.0, lot cells spread ventrally in heterozygous, but not in homozygous mutant embryos. (C,F) Expression of lacZ reporter gene that presumably reflects endogenous netrin 1 expression. lacZ is only expressed in the neuroepithelium of the GE at E12.5 (C), but the expression expands onto the surface of the rostral olfactory tubercle at E13.5 (F). (G,H) E14.5 telencephalons double-stained with mAb lot1 and X-gal. (I,J) High-magnification views of boxed areas in G and H. (K,L) Coronal sections of E14.5 telencephalon including the LOT stained with mAb lot1 (magenta) and anti-neuropilin 1 antibody (green). The distribution of lot cells expands ventrally towards the lacZ-expressing region in the olfactory tubercle in the heterozygous telencephalons (arrow in I) but not in the homozygous telencephalons, where lot cells seem to be stacked in the ventral edge of the cellular array (arrowheads in J,L). All of 30 homozygous mutants showed the same disruptive phenotype. Scale bars: 500 µm.





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