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Figure 2


Fig. 2. Mis-expression of shh results in an increase of the MDT. shh mRNA (150 pg) was injected into one of 32 cells to generate randomly distributed shh-positive cell clones, shown by an FITC-coupled lineage tracer (A-A''). Mis-expression of shh leads to expansion of ptc1 (B-C', bracket) and induces ptc1 positive clones at ectopic sites (arrows). dlx2a as well as lhx5 are expanded following shh mRNA mis-expression (D-G', bracket). dlx2a is induced anterior to the ZLI (white arrow), but not posterior (blue arrow). dbx1a and emx2 expression increases in size after shh mRNA mis-expression (H-K', brackets).





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