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Fig. 3. Apoptosis and proliferation in ß-catenin CKO embryos.
(A) Whole-mount anti activated caspase 3 staining in E8.5 embryos.
Increased numbers of activated caspase 3 cells detected mainly in the anterior
(A) region of the otic ectoderm in CKO (arrowhead). Brackets indicate the
position of the contralateral otic placode. Broken lines show the first (1p)
and second (2p) branchial pouches. Sections of anterior and posterior parts of
the otic region are shown stained with activated caspase 3 (green), DAPI
(magenta) and processed with a probe to Foxi2. Apoptotic cells are
observed in unthickened, Foxi2+ ectoderm (arrowheads) in
CKO embryos but are not seen in controls. No capase 3 cells can be seen in the
thickened, Foxi2- otic placode (brackets). (B)
Average number of activated caspase 3-expressing cells per section in the otic
region (indicated as rectangles in A). (C) Dividing cells (green,
arrowheads) marked by phospho-Histone H3 (pH3) at E8.75 co-stained with
ß-catenin (red) and DAPI (blue). (D) Average number of the
pH3-positive cells per section in the otic cup at E8.75 shows a reduction in
dividing cells in the CKO otic cup. Numbers are normalized to the length of
the apical surface of the placode (see Materials and methods). Scale bars: 100
µm.
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