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Fig. 7. Upregulation of pre-placodal markers correlates with Fgf
responsiveness. (A) Anterior epiblast was isolated from HH stage
3+-4 quail embryos and grafted into chick hosts at 0-4 ss. Grafts
were placed either adjacent to the neural plate at the level of the
presumptive trigeminal placode, or lateral to the presumptive trigeminal
placode. Grafts were removed after 2 or 8 hours and cultured for a further 12
hours in the presence or absence of 50 ng/ml Fgf2. Control pieces of epiblast
were isolated and cultured in Fgf2 for 20 hours without first being grafted
into chick hosts. (B) Pax2 expression in cultured explants of
transplanted quail epiblast, detected by QCPN expression. Pax2 is induced only
by Fgf2 in explants grafted into the pre-placodal region for 8 hours. Two
representative explants are shown for this condition, separated by broken
lines. (C) Epiblast does not express Eya2 or label with
pan-Dll antibodies after 20 hours of culture in the presence or absence of
Fgf2.
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