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Fig. 1. Isolation of secreted molecules from the Hydra head organizer
in a yeast signal peptide secretion screen. (A) Polyps of the
temperature-sensitive strain Hydra magnipapillata sf-1 were exposed
to heat shock for 3 days, causing the elimination of interstitial cells.
Heads, as well as regenerating tips, were isolated from the heat-shocked
polyps at various times after head removal for mRNA and cDNA synthesis.
(B) Cloning strategy. Size-fractionated cDNA was cloned adjacent to a
signal peptide-deficient yeast invertase gene for expression in the
yeast strain YTK12. Clones expressing a fusion protein with an intact
Hydra signal peptide were selected by growth on raffinose plates.
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