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Figure 8


Fig. 8. Heterologous expression of hydkk1/2/4 in Xenopus laevis embryos. (A) Overexpression of xdkk1 or hydkk1/2/4 induces the Dickkopf phenotype. (B) Inhibition of xwnt8-mediated secondary axis induction by xdkk1 or by hydkk1/2/4 co-injection. (C) Animal cap assay. Inhibition of siamois induction by co-injection of xdkk1 or hydkk1/2/4 was assayed by RT-PCR. WE, whole embryos; xbra, Xenopus brachyury; h4, histone-4. (D) Co-injection of hydkk1/2/4 (750 pg and 3 ng) or xdkk1 blocks xwnt8-induced activation of a siamois-luciferase reporter construct (stage 10 to 10.5).





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