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Figure 2


Fig. 2. Fate mapping of Notch1 ablated retinal cells at embryonic timepoints. (A) Schematic illustration of transgenic constructs. The floxed Notch1 allele was generated by flanking the first coding exon with LoxP sequences. Removal of exon1 of Notch1 with cre recombinase removes the exon encoding the signal peptide and leads to the generation of a null allele. ROSA26-R (R26R) is a cre recombinase reporter comprising a LoxP flanked stop codon preceding a ß-galactosidase coding region (lacZ). The Chx10-CRE mice contain a BAC transgene consisting of a cre-GFP fusion knocked into the Chx10 promoter. Notch1-ablated retinae were generated by crossing the Chx10-CRE allele into Notch1 flox/flox mice. Fate mapping of recombined cells was possible by X-gal staining in mice additionally containing the R26R allele. (B-E) Fate mapping of wild-type (B,C) and Notch1 ablated (D,E) retinal progenitor cells at E16.5 (B,D) and E18.5 (C,E), as detected by X-gal staining. INBL, inner neuroblastic layer; ONBL, outer neuroblastic layer.





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