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Figure 2


Fig. 2. Wg signaling regulates DE-Cad levels and epithelial cell-cell adhesion in wing imaginal discs. AJs are visualized by DE-Cad immunolocalization (red or grey, A-E',I-I') and F-actin staining (red or grey, F-G'). GFP (green) marks the domains where transgenes are misexpressed. (A,A') Somatic clones expressing Dsh display upregulation of DE-Cad and reduction in apical cell circumferences in their AJs (arrowheads in A). Inset displays XZ section of a Dsh-expressing clone (green) to reveal cell-autonomous upregulation of DE-Cad in the AJs. (B-D') Somatic clones expressing Wg display both autonomous and non-cell autonomous (arrowheads in B,B') upregulation of DE-Cad. (C,C') Magnified image of the clone shown in the boxed areas (B,B'). Broken line indicates the border of the Wg-expressing clones. DE-Cad level within the clone (red broken line in C') is higher than in the neighboring cells. (D,D') A Wg-expressing somatic clone in the hinge region of wing imaginal disc also displays apical cell shape changes within and outside the Wg-secreting clones, besides epithelial misfolding. (E-G') Expression of GPI-DFz2 (en-Gal4 X UAS-GPI-DFz2) downregulates DE-Cad levels (E,E') and also F-actin (F-G') in the posterior (P, green) when compared with the anterior (A) wing compartment. (G,G') XZ optical section (along the yellow line in F) revealing downregulation of F-actin in posterior wing compartment; arrow indicates the AP boundary. (H-I') In wing imaginal disc of Nts mutant larva, grown at a restrictive temperature, expression of Wg at their DV boundary (arrowhead, H) is extinguished. These discs (I) also do not display characteristic PD gradient in the levels of DE-Cad (for wild-type pattern, see Fig. 1E). Higher magnification of the boxed region in I reveals nearly uniform apical cell shapes along the PD axis (I'). (J-M) Somatic clones overexpressing Dsh (J) or a non-degradable form of ß-catenin/Arm, ArmS10 (K) or DE-Cad (L) display smooth clone borders, while those of clones expressing wild-type form of ß-catenin/Arm, ArmS2 (M) are `wiggly'.





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