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Fig. 4. Ft regulates cell-cell adhesion and DE-Cad expression. ft mutant clones (A-C) are marked by loss of GFP (green). Discs are stained for DE-Cad (red or grey in B-B'',D-E') or DE-Cad-lacZ (red or grey, C,C'). (A) ft⁴²² mutant clones display altered cell-cell adhesion with characteristic smooth clone borders, unlike the `wiggly' borders of their wild-type (ft⁺/ft⁺) twins (arrowheads, brighter green). (B-B'') In ft⁴²² clones, DE-Cad is upregulated. Part of this clone (box in B) is shown at a higher magnification (B'') to reveal higher levels of DE-Cad in the AJs of cells lacking Ft (ft^-/ft^-) when compared with those of the neighboring wild-type (ft⁺/ft^-) cells; broken green line marks the clone border. (C,C') ft^fd clones display upregulated expression of DE-Cad-lacZ. (D-E') Misexpression of Ft (enGal4/UAS-ft) in the posterior (P) wing compartment (GFP, green) downregulates DE-Cad when compared with that in the anterior (A) wing compartment. Boxed area in D is shown at higher magnification in E,E'. The PD gradient is lost in cell shape in the posterior wing compartment where Ft is overexpressed (see Fig. 1E); arrowheads mark the DV boundary.

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