QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Pancreas development is dependent on RAR function. (A) rara2a expression in dorsal view at 10 hpf. (B) rara2b expression in dorsal view at 10 hpf; arrowhead indicates the approximate AP position where the pancreas will develop. (C) insulin is expressed at 24 hpf following morpholino knock-down of RAR2b (indistinguishable from controls; data not shown). (D-F) rara2b expression in endoderm cells; examples are indicated by asterisks. Confocal images of rara2b expression (red) at 10 hpf, SOX32-positive donor-derived endoderm is labeled with fluorescein (green). Approximate area of image is boxed in B. (G) DN-RAR2a mRNA blocks insulin expression in a dose-dependent fashion (concentrations injected in ng/µl). (H) Histogram showing number of insulin-expressing cells in uninjected control embryos (n=44) compared with embryos injected with RAR2a-MO (n=11), RAR2b-MO (n=12), RAR-MO (n=12), RAR2a-MO plus RAR2b-MO (n=10), RAR2b-MO plus RAR-MO (n=7), DN-RAR2a mRNA (125 ng/ml; n=11) and DN-RAR2a mRNA (125 ng/ml) plus RAR2b-MO (RAR knock-down, RKD; n=85). Error bars indicate standard deviation. (I) Co-injection of DN-RAR2a mRNA and RAR2b-MO blocks insulin expression.

Return to article