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Fig. 5. The trachea specific overexpression of activated forms of DAAM and
Dia. Btl-Gal4 driven expression of C-DAAM (activated DAAM) affects the
cuticle pattern (A) and impairs actin organization in the tracheal
system (B). Fusion cells are narrow doughnut-shaped cells that are
easily distinguished from the regular tracheal cells by their spotted cuticle
pattern (C). Actin in fusion cells (red) is also concentrated into a
spotted pattern (D). When C-DAAM is ectopically expressed in the fusion
cells, it often induces a change in their shape, and leads to a partial
transformation of the spotted cuticle towards the taenidial fold pattern
(E). Additionally, we detected a strong actin accumulation in the
fusion cells where short actin bundles are often visible (F).
DE-cadherin labels the cell boundaries on B,D,F; arrows indicate the fusion
cells on D and F. C and D are the same wild type larval trachea, E and F are
from the same DAAM overexpressing trachea. Whereas, C-DAAM expression in the
tracheal network does not significantly alter the general structure of the
tubular system (G), the presence of DiaCA (an activated form
of diaphanous) leads to fusion defects in the dorsal trunk (black
arrow), constrictions at the fusion cells (green arrowhead), widening at other
areas of the dorsal trunk (black arrowhead) and impairments of terminal branch
differentiation (green arrow) (H). The tracheal system in G and H is
visualized by the luminal antibody 2A12. Scale bars: 50 µm in B for A,B;10
µm in D for C,D; 10 µm in F for E,F; 10 µm in G,H.
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