spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Functional and compositional analyses of MTJs. (A-D) Evans blue analysis reveals normal MTJ integrity in a control mouse diaphragm (A), but evidence of damage is observed in Lamb2-/- (B) and Lamb2-/-; MCK-B2 (C) mice at 3 weeks of age. A diaphragm from an older adult Lamb2-/-; MCK-B2; NEPH-B2 mouse shows no damage (D), suggesting eventual repair of MTJs. (E,F) Double staining for laminin ß2 (green) and laminin {alpha}2 (red) in intercostal muscles reveals concentration of ß2 at MTJs in both control (E) and Lamb2-/-; MCK-B2 (F) mice. (G,H) Double staining for mouse plus rat ß2 (G) and rat ß2 only (H) in a Lamb2+/-; MCK-B2 mouse shows that many MTJs contain little if any transgene-derived protein. (I-L) Double staining for total laminin ß2 (green) and {alpha}2 (red) in intercostal muscles from aged control (I,J) and Lamb2-/-; MCK-B2; NEPH-B2 (K,L) mice reveals efficient accumulation of ß2 at MTJs. Transgene-derived ß2 is missing from extra-junctional BMs in rescued mutant muscle fibers (F,K). Scale bars: in D, 1 mm for A-D; in H, 50 µm for A-I,K; in L, 20 µm for J,L.





Right arrow Return to article