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Figure 1


Fig. 1. Conditional inactivation strategy. (A) Generation of Tbx1 wild-type (WT), Tbx1 hygro, Tbx1 flox and Tbx1 null alleles. (B) Correct targeting was confirmed through Southern blot hybridization with a probe distal to the targeting vector integration site, indicated by the red bar in A. The restriction enzymes used were Acc65I and NotI, and the expected sizes of the different alleles are indicated in A. (C) PCR amplification was used to genotype the Tbx1 hygro/+ (lane 6; PCR1), Tbx1 flox/+ (lanes 2 and 3; PCR2) and Tbx1 null/+ (lanes 4 and 5; PCR3) mice. The position of the primers is indicated in A.





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