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Figure 2


Fig. 2. Tbx1 expression compared with Cre expression. Tbx1 expression in wild-type (C,D,G,I,K) and homozygous conditional mutant mice (E,F,H,J,L) at E8.5 (C,E) and E9.5 (D,F-H), compared with Cre expression in Foxg1-Cre mice at E8.5 (A) and E9.5 (B). Coronal vibratome sections of whole mounts from D (wild type) and F (mutant) are shown in G and H, respectively. Note Tbx1 expression in the core mesenchyme (arrows) of both wild-type (G) and mutant embryos (H). The wild type also shows expression in the pharyngeal pouch endoderm (arrowhead in G). Immunohistochemistry with a polyclonal Tbx1 antibody on E11.5 sagittal sections confirms the presence of the Tbx1 protein in the splanchnic mesenchyme (arrow) of the mutant (J) and wild-type (I) embryos. Radioactive ISH on E11.5 sagittal sections shows Tbx1 expression in the otic vesicle (ov) and the adjacent splanchnic mesenchyme (K). The OV expression of Tbx1 is absent from the conditional null embryo (L), although the mesenchymal staining remains intact. (M-O) X-gal staining of E9.0 (O) and E9.5 (M,N) sagittal (M) and transverse (N,O) sections of ROSA26/Foxg1-Cre progeny. Cre activity is evident in the pharyngeal pouches (pp) and the otic vesicle (ov). (P-U) Tbx1 expression by ISH on coronal (P,Q) and sagittal (R-U) sections in control (P,R,T) and conditional null embryos (Q,S,U). Scale bars: 100 µm in E,H,N,Q,S,U; 0.2 mm in F; 0.5 mm in J,K. Asterisks indicate core mesoderm; ov, otic vesicle; pa, pharyngeal arch; pp, pharyngeal pouches.





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