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Fig. 3. Contribution of the somite to the vascularization of the host and to
formation of the smooth muscle tunicae. (A) E6.5 chick host, 5 days
after the graft. Cross-section at the trunk level. Triple labeling with QH1
(green)/MEP21 (red)/DAPI (blue). The vascular networks of the neural tube,
dorsal root ganglia, dermis, mesonephros, body wall and limb bud derive
entirely from the graft. Hematopoiesis has ceased at that stage. The MEP21
immunoreactivity with the coelomic epithelium around the lungs and the heart
is a staining artifact. Scale bar: 200 µm. Inset: closer view of the right
side of the aorta. The aortic endothelium on the grafted side mostly derives
from the graft. Scale bar: 50 µm. (B) E5 chick host, 3.5 days after
a bilateral graft of segmental plates. QH1 immunofluorescence overlaid with
Nomarski's interferential contrast. Wing bud level. The quail perineural
vasculature is conspicuous. ECs of the aorta are entirely quail. Quail ECs are
not found in internal organs. Scale bar: 150 µm. (C) Section
immediately adjacent to B. Double QCPN (red)/
SMA (blue)
immunofluorescence. QCPN shows numerous quail cells that surround the
notochord and the aorta. Quail cells closest to the aortic lumen express
SMA (arrows). Many QCPN+/SMA- cells
organize around the SMA+ layer. Scale bar: 70 µm.
(D-F) Detail of the ventrolateral side of an E4.5 chick aorta, 3 days
after a unilateral graft. QH1 (D) and SMA (E) immunohistochemistry
merged to Nomarski's interferential contrast. (F) Overlay of QH1 and
SMA signals merged to the Nomarski's picture. The endothelial (green)
and smooth muscle (blue) expression domains are clearly separated. The
arrowhead indicates the same endothelial cell. Scale bar: 30 µm. H, heart;
L, lungs; WB, wing bud.
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