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Fig. 3. Molecular structure of sor-1 and its predicted
gene product. (A) sor-1 was mapped genetically to the
region of LG III between unc-32 and sma-3. sor-1 is SL1
trans-spliced. The intron/exon boundaries were confirmed by sequencing the
cDNAs yk526e9 and yk336g5 and the RT-PCR products. (B) The protein
sequence of SOR-1. Mutant residues in sor-1(bp1), sor-1(bp2) and
sor-1(bp3) are shown in red. (C) Direct RNA binding by SOR-1.
Various SOR-1 protein fragments were incubated with the radiolabeled RNA
(5'UTR of egl-5, C08C3 nucleotides 41280-41840) and binding was
assessed by EMSA. Protein-RNA complex is marked by bracket. (D) Binding
of SOR-1(443-530) to RNA at a titrated protein concentration (ng/µl).
Binding of RNA by SOR-1 is competed by unlabeled ssRNA (10x), dsRNA
(10x) and partially by tRNA (100x), but not by DNA (10x and
100x refer to mass excess). The ssRNA, dsRNA and DNA competitors are
derived from T08D10 (nucleotides 7800-8408). Similar results were obtained
using several other competitors, including the ones derived from Y110A7A
(nucleotides 59588-60325) and Y113G7B (nucleotides 81290-82026). Thirty
ng/µl of proteins (final concentration) were used for each RNA-binding
reaction unless otherwise noted for the titration experiments.
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