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Figure 4


Fig. 4. Colocalization of SOR-1 and SOP-2 in nuclear bodies. (A) Expression of sor-1::gfp in the hypodermal nuclei of adult animals. SOR-1::GFP is distributed throughout the nuclei with accumulation in distinct nuclear speckles. (B) Expression of SOR-1 in an early embryo. Polyclonal antibodies against SOR-1 coupled with rhodamine-conjugated goat anti-rabbit IgG were used to detect expression. SOR-1 is localized in distinct nuclear bodies (arrow). (C-E) Localization of SOP-2::GFP (C) and SOR-1 (D) into nuclear bodies. (E) The superimposable confocal images of SOR-1 (red) and SOP-2::GFP (green) shows that they colocalize (yellow). (F,G) Unaltered localization of SOP-2 into nuclear bodies in sor-1(RNAi) mutant embryos (F). Absence of staining of SOR-1 in sor-1(RNAi) mutant embryos confirms the specificity of the antibody (G). (H-J) The merged images of SOP-2 (H) and DAPI (I) shows that SOP-2(bx91) is localized in cytoplasm in sop-2(bx91) mutant embryos (J). (K-M) The merged images of SOR-1 (K) and DAPI (L) shows that SOR-1 is localized in cytoplasm in sop-2(bx91) mutant embryos (M). (B-M) Single confocal sections.





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