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Figure 1


Fig. 1. Reduced NF-{kappa}B activity in skin of cI{kappa}B{alpha}{Delta}N and downless mice, but normal perinatal epidermal keratinocyte differentiation in cI{kappa}B{alpha}{Delta}N mice. (A) Total protein extracts of newborn wild-type and cI{kappa}B{alpha}{Delta}N ({Delta}N) skin were analysed for I{kappa}B{alpha}{Delta}N expression in a western blot using an anti-I{kappa}B{alpha} antibody (lower panel). Mouse embryonic fibroblasts isolated from cI{kappa}B{alpha}{Delta}N mice were used as a positive control ({Delta}N MEF, right lane). ß-Catenin protein levels remained unchanged in all extracts (upper panel). ns, non-specific. (B) EMSA of total skin extracts of newborn wild-type, cI{kappa}B{alpha}{Delta}N ({Delta}N) and downless (dl) skin. Extracts were treated with specific antibodies against NF-{kappa}B p65, p50 and RelB as indicated, which inhibited ({alpha}-p65) or upshifted ({alpha}-p50) the DNA-binding complex. No effect was seen with RelB. Strong p50 homodimer binding is present in skin extracts. (C) Sagittal cryosections of E17.5 and P0 wild-type and cI{kappa}B{alpha}{Delta}N ({Delta}N) embryos were incubated with antibodies to different epidermal differentiation markers (AP substrate, in red), as indicated above (loricrin, involucrin, filaggrin, keratin 10). Counterstaining was carried out with Mayer's haemalaun (blue).





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