DEV02281 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Neural crest cells migrate superior to the stomodeum in smo^- embryos. (A,C) Kaede protein was photoconverted at 12 hpf in wild-type (A) or smoh^- (C) embryos. (B,D) At 18 hpf, neural crest cells had migrated dorsal to the stomodeum (arrow) in both wild-type (B) and smo^- (D) embryos.

• Supplemental Figure 2 -

  Fig. S2. Expression of shh is maintained in presumptive endoderm but not presumptive stomodeum of smoh^- embryos. (A,B) Cross-sections taken at the posterior edge of the eye (arrowhead) show shh is expressed in presumptive stomodeum of wild-type (A, arrow), but not smoh^- (B) embryos. (C,D) More posterior sections, still through the first arch, show shh is expressed by presumptive medial endoderm in both wild-type (C, arrow) and smoh^- (D, arrow) embryos.

• Supplemental Figure 3 -

  Fig. S3. Epithelial integrity of the stomodeum is intact in smo^- embryos. Epithelial markers p63 (A,B) and pan-cadherin (C,D) are expressed in the stomodeum of both wild-type (A,C) and smo^- embryos (B,D).

• Supplemental Figure 4 -

  Fig. S4. Rescue of anterior craniofacial outgrowth by wild-type stomodeum in smo^- embryos. (A,B) Confocal images show outgrowth of the developing anterior craniofacial domain (arrow) in 48 hpf wild-type (A) and smo^- embryos receiving wild-type stomodeum transplants (B). (C) No outgrowth is evident in smo^- embryos not receiving transplants.

• Movie 1 -

  Movie 1. Wild-type first arch crest cell migration and condensation.

• Movie 2 -

  Movie 2. Failure of crest cells to condense on the stomodeal roof in smo mutants.

• Movie 3 -

  Movie 3. Anterior craniofacial crest cells migrate posterior to the eye in smo^- embryos.

• Movie 4 -

  Movie 4. Wild-type crest cells fail to condense on the stomodeal roof and migrate posterior to the eye in smo^- hosts.