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Fig. 2. smo- mutant embryos have no anterior neurocranium
precartilage condensations. Embryos were stained with RNA probe to the
precartilage marker sox9a, the pharyngeal arches were dissected away
from the embryos and ventral views of the neurocranium were imaged. (A)
At 48 hpf, sox9a-positive cells prefigure the morphology of the 4 dpf
neurocranium in wild-type embryos. (B) Embryos lacking all Hh signaling
have a complete loss of anterior neurocranium precartilage condensations;
however, immediately posterior precartilage condensations are present (arrows
in A,B). EP, ethmoid plate; TR, trabeculae; WT, wild type. Anterior is
leftwards. Scale bar: 50 µm.