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Fig. 4. Pre- and postmigratory anterior neurocranium and upper jaw precursors
co-localize. Single or two adjacent neural crest cells were labeled via
microelectroporation at 12 hpf (A-D) or 24 hpf (E-H, Meckel's cartilage and
palatoquadrate data reanalyzed from Crump et al. unpublished). (A-D) At
12 hpf, pterygoid process (C, arrows, n=2) and anterior neurocranium
(D, arrow, n=14) precursors are localized to a discrete cluster
postoptically (B). (E-H) Within the first arch, at 24 hpf, pterygoid
process (G, arrow, n=8) and anterior neurocranium (H, arrow,
n=7) progenitors co-mingle on the stomodeal roof, away from Meckel's
cartilage (n=5) and palatoquadrate (n=16) precursors. The
condensed anterior craniofacial neural crest precursor domain is indicated by
arrowheads in E and F. (B,F) Schematic enlargements of A and E showing
compiled fate mapping data for 12 hpf (B) and 24 hpf (F). Anterior
neurocranium, yellow; pterygoid process, olive; non-pterygoid palatoquadrate,
blue; Meckel's cartilage, red. Black dots in B represent fates other than 4.5
dpf skeletal elements. EP, ethmoid plate; MC, Meckel's cartilage; NC, neural
crest; pPQ, non-pterygoid palatoquadrate; PTP, pterygoid process of the
palatoquadrate; TR, trabeculae. Anterior is leftwards in all panels.
(A,B,D-F,H) Dorsal is upwards. (C,G) Dorsal views. Scale bar: 50 µm.
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