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Fig. 5. Ascl1 is required cell autonomously to promote pit1 expression,
cell survival and Prl production. (A-C) Lateral views on chimeric
embryos at 26 hpf embryos, in situ hybridized for pit1 transcripts in
blue and with transplanted wild-type cells in brown (indicated with arrows).
(A) Wild-type control recipient with wild-type donor cells in the
telencephalon and normal pit1 expression. (B) pia recipient
with wild-type cells in eyes, telencephalon and ventral regions of
diencephalon, lacking adenohypophyseal pit1 expression. (C)
pia embryo with few wild-type cells within adenohypophyseal domain,
showing pit1 expression in these wild-type cells only. (D-F)
Frontal views of pituitaries at 32 hpf. Nomarski images superimposed with red
fluorescence revealing transplanted cells; green fluorescence revealing
Acridine Orange (AO)-positive cells. (D) Wild-type control recipient with
AO-negative wild-type donor cells in the pituitary. (E) pia
recipient, with AO-negative transplanted wild-type cells (red), but many
AO-positive host cells (green) in pituitary. (F) Wild-type recipient, with one
AO-positive (yellow) and two AO-negative ascl1a morphant donor cells
in pituitary. (G-I) Ventral views of pituitaries at 48 hpf, anterior
towards the left. Left panels show transplanted h2a::h2a-GFP
transgenic cells weakly labeled in green, right panels an overlay of the same
images with anti-Prl immunostaining in red. (G) Wild-type host; (H)
pia host without transplanted cells in pituitary; (I) pia
host with three wild-type cells in pituitary, two of which produce Prl
protein.
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