|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Differential allocation of N-cadherin to the apical daughters of
perpendicularly dividing DM progenitors. (A) Transverse section
showing the DM and underlying myotome (M) co-stained with nuclear Hoechst
(blue) and N-cadherin (green). Cell divisions occur at the apical portion of
the DM (arrowhead). (B-E) High magnifications of dividing cells stained
with Hoechst (blue), N-cadherin (green) and 
-tubulin to label
centrosomes (red). (B) A cell dividing parallel to the mediolateral axis of
the DM. Both daughter cells retain N-cadherin staining at their apical
aspects. (C,D) Perpendicularly dividing DM cells in which only the apical
progeny remains N-cadherin positive. (E) Oblique mitosis showing a similar
segregation of N-cadherin to the apical daughter cell. In contrast to the DM,
myotomal cells express homogeneous N-cadherin on their surface. Panels in
second and third rows show, separately, Hoechst staining of nuclei associated
with -tubulin and N-cadherin with -tubulin. n, at least
60 mitoses were scored with equal N-cadherin distribution in progeny of all
parallel mitoses, and apically localized N-cadherin in all perpendicular
divisions. Scale bar: 33 µm in A; 10 µm in B-E.
|
