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Figure 3


Fig. 3. Maturation of ECN and LRN. (A-C) The distribution of EYFP-labelled neurons at the level of ECN (A), rostral LRN (B) and caudal LRN (C). Many labelled cells are located in deeper regions of the myelencephalon. Arrowheads in A indicate axons of presumptive LRN neurons. (D-G) Transverse sections of E18.5 mice myelencephalon after immunostaining for EYFP (green) and Mbh2 in situ hybridization (purple). Medial is towards the left and dorsal is upwards. Higher-magnification views of the regions marked d-g. EYFP-labelled cells extended several dendrite-like processes that indicated maturation and expressed Mbh2 mRNA (the right panels). Arrows in each of the two panels in E and F indicate the same cells. EYFP was electroporated into the left lRL. Cb, cerebellum; mLRN, magnocellular part of LRN; pLRN, parvocellular part of LRN. Scale bars: in C, 200 µm for A-C; in right panel of G, 50 µm for D,G and 20 µm for E,F.





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