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Fig. 5. Midline crossing and ventricle-directed migration of PGN and RTN
neurons. (A) EYFP-labelled cells tangentially migrating across the
midline of the pons in an E14.5 mouse. Arrowheads indicate radially deflected
cells. (B) At E15.5, some EYFP-labelled cells accumulated on the
ipsilateral side, while some others were still crossing the midline.
(C) EYFP-labelled cells that had turned to become ventricle orientated
before (arrowheads on the left) and after (arrowhead on the right) midline
crossing. E14.5. (D) Labelled cells within the pons. E15.5.
(E-G) High-power views of the rectangular areas marked e-g in D.
Ventricle-directed neurons extended a trailing and a leading process
(arrowheads in E and F). (F) A cell that had just changed its migratory
direction from tangential to ventricle (arrowheads). (G) An RTN neuron that
extended several dendrite-like processes. EYFP was electroporated
into the left lRL. Transverse sections. Dorsal is upwards. Broken lines in A-E
represent the positions of the midline. Scale bar: 50 µm for A-C; 100 µm
for D; 20 µm for E-G.
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