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Fig. 2. Rescue of gonad compaction. Expression of indicated FOI proteins in
foi-mutant embryos. (A) Graph indicates percentage of fully
compacted gonads, as judged by 
-VAS staining (average of two
independent lines +s.d.; asterisk indicates construct for which three
independent insertion lines were tested). (B-G) Confocal images of
representative stage 15-16 gonads immunolabeled with -VAS (germ cells,
red) and -ZFH-1 (SGPs, green). (B) Wild type, (C) foi mutant,
(D-G) foi mutants with the indicated HA-FOI proteins expressed. Only
expression of HA-FOI (D) or Y646A (G) rescues gonad compaction. Insets in C
and E represent the most severe phenotype observed for these two genotypes;
the full panels represent a less severe, but more representative, phenotype.
Scale bars: 10 µm.
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