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Fig. 3. Rescue of germ cell ensheathment. Expression of indicated FOI proteins in foi-mutant embryos. (A) Graph indicates percentage of gonads with ensheathed germ cells (as judged with ${alpha}$ -NRT). Only expression of wild-type HA-FOI fully rescues germ cell ensheathment. (B-G) Confocal images of representative stage 15-16 gonads immunolabeled with ${alpha}$ -NRT (SGP cell surface). Insets show location of germ cells ( ${alpha}$ -VAS, blue) relative to SGPs ( ${alpha}$ -NRT, red). (B) Wild type, (C) foi mutant, (D-G) foi mutants with the indicated HA-FOI proteins expressed. (F,G) Examples of gonads in which germ cell ensheathment is rescued in these genotypes (i.e. more ensheathment than observed in foi mutants). Arrows indicate examples of germ cell ensheathment. Perimeter of gonad is outlined. Scale bars: 10 µm.

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