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Figure 4


Fig. 4. Rescue of DE-cadherin expression. Expression of indicated FOI proteins in foi-mutant embryos. (A) Graph indicates the percentage of gonads with higher levels of DE-cadherin expression than foi-mutant controls. (B-G) Confocal images of representative stage 15-16 gonads, immunolabeled with {alpha}-DCAD2 (DE-cadherin). Insets show positions of germ cells ({alpha}-VAS, blue) relative to DE-cadherin ({alpha}-DCAD2, red). (B) Wild type, (C) foi mutant, (D-G) foi mutants with the indicated HA-FOI proteins expressed. Wild type (B), HA-FOI (D), T557P (F) and Y646A (G) gonads express DE-cadherin, while foi20.71 (C) and H554A (E) gonads do not. DE-cadherin expression in gonads expressing T557P (F) is more diffuse than in wild type (B). DE-cadherin in neighboring tracheal branches is labeled (tr). Perimeter of gonad is outlined. Scale bars: 10 µm.





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