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Fig. 2. Pulmonary immaturity in
Cebpa
/ mice.
(A) Lung sections prepared at E18.5 and stained with Hematoxylin and
Eosin revealed normal lung inflation and histology, indicated by saccular
structures lined by squamous type I cells and cuboidal type II cells, in
control littermates. (B) Severe atelectasis, pulmonary congestion,
reduced septation, thickened mesenchyme and columnar epithelium lining the
peripheral saccules were observed in the
Cebpa/ lung. Scale bar: 50 µm.
(C,D) Consistent with the observed immaturity in the
Cebpa/ lung, PAS staining demonstrated
increased glycogen content in the bronchiolar epithelium of
Cebpa/ mice (D) compared with control
littermates (C). Scale bar: 1 µm. Electron microscopy was performed on
lungs from control (E,F) and Cebpa/
(G,H) mice at E18.5. Squamous type I cells (black, arrowhead) and
cuboidal type II cells (white triangle) containing numerous lamellar bodies
(*), apical microvilli (MV) and highly organized rosette glycogen
were observed in the lungs of control mice. Lamellar bodies were observed in
the lumen of peripheral airspaces and secreted surfactant (thin arrow) was
identified in the airspaces (E). By contrast, lamellar bodies, secreted
surfactant, and type I cells were not observed in the lungs of
Cebpa/ mice (G). Cytoplasmic glycogen (GLY)
was dispersed and apical microvilli were smaller in epithelial cells lining
the abnormal lung tubules (H). Micrographs are representative of three
Cebpa/ mice and littermate controls.
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