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Figure 5


Fig. 5. Cardiac and lymph gland defects in Hand mutant larvae. (A-J) Three-dimensional reconstruction of confocal scans of Drosophila embryos and larvae carrying Hand-GFP as different stages. (A-C,G,H) Wild-type embryos and larvae; (D-F,I,J) Hand mutant embryos and larvae. Arrowheads indicate the positions of the lymph gland. (A-F) Hand mutant embryos and larvae display cardiac morphological defects compared with wild type. (G,I) Lymph gland hematopoietic progenitors are almost completely abolished in 24 hour AEL Hand mutant larvae (indicated by an arrowhead). (H,J) Three dimensional chamber-like structure is abolished in Hand mutant larvae at 26 hours AEL, accompanied by hypoplastic heart tube and dramatically reduced pericardial nephrocytes.





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