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Fig. 6. Rescue of larval cardiac and hematopoietic defects by P35 or human
HAND2. (A-F) Dorsal views of stage 16 embryos labeled with TUNEL (red) and
Hand-GFP (green). Compared to wild type (A,B), ectopic apoptosis (shown
by TUNEL staining) was observed in Hand mutants in the regions
normally occupied by lymph gland hematopoietic progenitors (indicated by an
arrowhead) and pericardial cells, with a few TUNEL positive cells found among
the cardioblasts (C,D). The ectopic apoptosis in Hand mutants
could be rescued by targeted expression of P35 (E,F). Targeted
expression of P35 also effectively rescued the cardiac and lymph gland defects
in Hand mutant embryos, as shown by Hand-GFP (F). In C-F, arrowheads
indicate the position of the lymph gland. At larval stages, Hand
mutant larvae rescued by targeted expression of P35 displayed cardiac and
lymph gland defects at 18 hours AEL (G). These defects become more
severe at 24 hours AEL (H). The cardiac and hematopoietic defects of
Hand mutants were more effectively rescued by targeted expression of
human HAND2 at 16 hours (I) and 24 hours AEL (J), and
completely rescued by targeted expression of Drosophila Hand (data
not shown).
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