|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. NANOG overexpressing cells adopt markers of primitive ectoderm
cells. (A) The ICM (inner cell mass) and epiblast (primitive
ectoderm) can be distinguished by the differential expression of a subset of
marker genes. ICM has a high expression of Rex1 and Gbx2,
and low expression of Fgf5. Epiblast has low expression of
Rex1 and Gbx2, and upregulation of Fgf5. (B)
Real-time RT-PCR analysis was performed on markers that distinguish between
ICM and primitive ectoderm cells. Expression was examined on wild-type cells
and on NANOG overexpressing clones (shown as the average of three
separate clones). Although GAPDH and OCT4 did not show a
significant change, REX1 and GBX2 were significantly
downregulated, and FGF5 was significantly upregulated following
NANOG overexpression (*P<0.05). (C)
Semi-quantitative RT-PCR analysis was executed on the markers described in B.
Expression was examined in wild-type cells, NANOG overexpressing
clones and revertant clones from which the transgene was excised. Shown are
representative pictures of the examined clones. (D) RT-PCR analysis was
performed on Nanog overexpressing MESCs. A comparison was performed
between two separate MESC lines (E14Tg2a and RF8) and clones overexpressing
Nanog derived from them.
|
