|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Sfrp1-/-;Sfrp2-/- embryos
display shortening of the thoracic region and limb morphogenesis
abnormality. (A) Gross morphology of
Sfrp1-/-;Sfrp2-/- mutant (left) and control
(right) embryos at E14.5. Arrows indicate the craniofacial abnormality;
arrowheads indicate edematous defects. Scale bar: 1 mm. (B-E) Cartilage
staining in control (B,D) and Sfrp1-/-;Sfrp2-/-
(C,E) embryos at E15.5. The number of thoracic vertebrae was reduced in
Sfrp1-/-;Sfrp2-/- embryos. Although vertebrae
numbers were normal, the sides of vertebrae were reduced in the lumbar and
sacral regions. Arrowhead, C7 vertebra; double arrowhead, T1 vertebra; single
arrow, L1 vertebra; double arrow, S1 vertebrae; C1, atlas. Scale bar: 1 mm.
(F-I) Extra digits (arrowhead) on the hindlimb of
Sfrp1-/-;Sfrp2-/- embryos (G) at E14.5, and the
skeletal pattern of the hindlimb in control (H) and
Sfrp1-/-;Sfrp2-/- (I) embryos at E15.5. T,
tail. Scale bars: 500 µm in F,G; 200 µm in H,I. (J,K)
Fgf8 expression in the limb bud of control (J) and
Sfrp1-/-;Sfrp2-/- (K) embryos at E10.5. Arrows
indicate the extra stripes of Fgf8 expression in the ventral surface
of the hindlimb bud. A, anterior; P, posterior. Scale bar: 400 µm.
|
