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Fig. 7. Perturbed expression of cyclic genes during somitogenesis in
Sfrp1-/-;Sfrp2-/- embryos.
(A-G) Altered Lfng oscillating cycles in the PSM of
Sfrp1-/-;Sfrp2-/- embryos at E8.5. The
Lfng cycle was examined in control (A,D) and
Sfrp1-/-;Sfrp2-/- (B,C,E,F) embryos by culture
of half of the posterior region for different lengths of time [A, B and C, 0
and 60 minutes (t=0', 60'); D, E and F, 0 and 75 minutes
(t=0', 75')]. The dagger indicates a strong extra stripe in the
anterior-most region of the PSM. Scale bar: 250 µm. (G) Schematic diagram
of Lfng expression in control and
Sfrp1-/-;Sfrp2-/- explants. Traveling stripes
of Lfng expression are indicated by numbers. (H,I) Oscillation
pattern of Lfng in control (H) and
Sfrp1-/-;Sfrp2-/- (I) explants at E9.5. Half of
the posterior region was cultured for either 0 (t=0') or 75
(t=75') minutes. Scale bar: 250 µm. (J-U) Expression
of Hes7, Axin2 and Nkx1 in the PSM of
Sfrp1-/-;Sfrp2-/- embryos. Half of the
posterior region was cultured for either 0 (t=0') or 75
(t=75') minutes. (J-O) Hes7 expression in control
(J,M) and Sfrp1-/-;Sfrp2-/- (K,L,N,O) explants
at E8.5. (L,N,O) Hes7 expression was perturbed in a large proportion
of Sfrp1-/-;Sfrp2-/- explants. (K) Nearly
normal Hes7 expression cycles were observed in
Sfrp1-/-;Sfrp2-/- explants at lower frequency.
The dagger indicates a strong extra stripe in the anterior-most region of the
PSM. Scale bar: 250 µm. (P-R) Dynamic expression of Axin2 in
control (P) and Sfrp1-/-;Sfrp2-/- (Q,R)
explants at E8.5. An arrowhead indicates the higher expression domain of
Axin2 in the primitive streak. Stripes of Axin2 expression
were difficult to identify even in control explants at earlier embryonic
stages. Scale bar: 250 µm. (S-U) Expression of Nkd1 in control (S)
and Sfrp1-/-;Sfrp2-/- (T,U) explants at E8.5.
Scale bar: 250 µm.
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