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Figure 5


Fig. 5. Phenotypic analysis of ph mutant clones through puparium formation. Composite confocal images of mosaic fly brains fixed during puparium formation (0D APF), 1D APF or 2D APF. In wild-type mosaic brains, GAL4-NP225 constantly labeled clones of projection neurons through pupal stages (A-C). By contrast, GAL4-NP225 labeled many ectopic clones of ph mutant neurons after 2D APF (D-F). Genotypes: (A-C) FRT19A/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/GAL4-NP225; (D-F) FRT19A,ph602/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/GAL4-NP225. Scale bar: 20 µm.





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