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Fig. 6. Ecdysone-dependent transformation of ph mutant neurons in
cultured mosaic larval brains. Composite confocal images of variably
cultured larval brains that contain clones of ph mutant neurons with
GAL4-OK107 as a driver. Few GFP-positive cells exist after 4 days of
culture in the absence of 20-hydroxyecdysone (A). By contrast, there
are many GFP-positive cells when ecdysone was added at the beginning
(B) or on the fifth day of the culture (C). Genotype:
FRT19A/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/+;GAL4-OK107/+. Scale
bar: 20 µm.
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