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Figure 8


Fig. 8. Derepression of abd-B in clones of ph mutant neurons. Composite confocal images of whole wandering larval CNSs (A,H) and MARCM clones of wild-type (B-D,I-K) or ph mutant (E-G,L-N) neurons in the central brains of wandering larvae. Green shows MARCM clones that are positive for mCD8-GFP (B,E,I,L), red shows anti-Abd-B (A,C,F) or anti-Ubx (H,J,M). (D,G,K,N) Merged images. (A-G) Endogenous Abd-B is restricted to the terminal one-third of the ventral ganglion (A), and ectopic expression of abd-B in MARCM clones of ph mutant neurons (compare F and G with C and D). (H-N) By contrast, endogenous Ubx is enriched in the middle segment of the ventral ganglion (H) and no ectopic expression of Ubx is detected (I-N). Genotypes: (A,H) wild type; (B-D,I-K) FRT19A/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/+;elav-GAL4/+; (E-G,L-N) FRT19A,ph602/FRT19A,hs-FLP,tubP-GAL80;UAS-mCD8-GFP/+;elav-GAL4/+. Scale bar: 20 µm.





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