(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 5. ESD1 encodes ARP6. (A) Scheme of the ARP6
gene showing the position of the T-DNA insertion in the esd1-10
mutant. Exons are shown as black boxes. The position of the start and stop
codon are indicated. (B) Sequence comparison of AtARP6 with yeast (Sp),
C. elegans (Ce), Drosophila (Dm) and human (Hs) ARP6s, and
Arabidopsis Actin2. Amino acid residues in black are functionally
similar in all sequences and those in gray are similar in at least four of
them. Boxed regions indicate the two peptide insertions in ARP6s, which do not
disrupt the conserved actin fold structure. GenBank Accession numbers are
NP_566861 for AtARP6, AAF4849 for Dm ARP6, AAK14934 for Hs ARP6, CAA19116 for
Sp ARP6, NP_495681 for Ce ARP6, and BAB01806 for AtACT2. (C)
APR6 expression in different organs of Col plants. RT-PCR assays were
performed with RNA prepared from different tissues. R, roots; S, main stems;
F, flowers; FB, flower buds; CL, cauline leaves. RT-PCR products were blotted
and hybridized with a specific probe for ARP6. UBQ10 was used as a
loading control.
