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Fig. 7. Effect of esd1 mutation on histone H3 acetylation and
methylation in the FLC genomic region by ChIP analysis.
(A) FLC genomic region analyzed by ChIP. The white box
corresponds to the promoter FLC region, gray boxes to exons and the
black box to the first intron. The six FLC fragments analyzed by
semi-quantitative PCR are depicted and numbered. (B) PCR products after
25 cycles of esd1-2, fve-1 and esd1-2 fve-1 mutants, using
as a template DNA purified from chromatin inmunoprecipitated with antibodies
against acetylated H3 (AcH3). UBQ10 was amplified during 22 cycles
and used as control for DNA quantification. Fold enrichment in H3 acetylation
of fve-1 over esd1-2 and esd1-2 fve-1 double mutant
is shown. (C) PCR products after 25 cycles of Col, esd1-3, FRI,
esd1-3FRI, Ler, esd1-2, fca-1, esd1-2 fca-1, fve-1, and
esd1-2 fve-1 plants, using as a template DNA purified from chromatin
inmunoprecipitated with antibodies against acetylated H3 (AcH3).
UBQ10 was amplified during 22 cycles and used as control for DNA
quantification. Fold enrichment in H3 acetylation of mutants over wild-type
ecotypes is shown. (D) PCR products as in C, but using a as template
DNA purified from chromatin inmunoprecipitated with antibodies against
trimethylated H3-K4 (MeH3-K4). Fold enrichment in H3-K4 methylation of mutants
over wild-type ecotypes is shown.
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