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Fig. 1. Identification of chick genomic regions containing
Krox20 cis-regulatory elements. (A) Schematic
representation of the chick Krox20 locus, BAC clones and subfragments
tested by mouse transgenesis. Distances are relative to the start site of
transcription of the Krox20 gene. The BACs (constructs 1 and 2) and
the 65 kb fragment (construct 4) were injected alone and transgenic embryos
were analysed by in situ hybridization with a chick Krox20 probe. The
42 kb and 30 kb fragments (constructs 3 and 5), which do not carry the
Krox20 gene, were co-injected with a reporter fusion gene,
Krox20/lacZ, and transgenic embryos were analysed for
ß-galactosidase activity by X-gal staining. For each construct, the table
indicates the number of E8.5 transgenic embryos obtained (n) and the number of
embryos positive in r3 and r5 (r3/r5), or in the r3 to r5 region (r3-r5).
(B-E) Dorsal views of embryos transgenic for the indicated constructs
and analysed as indicated in A. Embryos are rostral side upwards. r,
rhombomere; ov, otic vesicle.
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