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Fig. 1. Disruption of sem-5 results in ectopic membrane protrusions
(EMEs). (A) An illustration of the BWMs (yellow and brown) and DA
and DB commissural motor neurons (blue) of the left side of C.
elegans. Both the outer row (brown) and the inner row (yellow) of BWMs
express Mb::YFP from the trIs10 integrated array, whereas only select
BWMs of the outer row (brown) express Mb::YFP from trIs30. The boxed
area indicates the area of the worm seen in D-I. (B) A cross-section of
A, with the dorsal, ventral and lateral hypodermal ridges (HR) indicated.
(C) Disruption of sem-5 by RNAi or hypomorphic
loss-of-function mutations results in EMEs as visualized with trIs10,
as does sem-5(RNAi) in the background of an different muscle reporter
strain RP168 [trEx(C26G2.1p::dsred2; B0285.6::mb::yfp)]
(Dixon and Roy, 2005), denoted
as trEx. Colored asterisks indicate significant differences
(P<0.001) versus the relevant controls: black, versus
trIs10;Ø(RNAi); red, versus trIs10; blue, versus
trEx;Ø(RNAi). n>80 for each genotype. Error bars represent
the standard error of the mean (s.e.m.). (D,E) The lateral BWM
membrane of trIs10 worms fed upon Ø(RNAi) is not
disrupted (blue arrow in D) and commissural motor axon guidance is normal (red
arrow in E). The unc-129nsp::cfp promoter also drives expression of
CFP in the hypodermal seam cells (orange arrows in E,I). (F,G)
trIs10; unc-5(e53) worms extend muscle arms (yellow arrow in F)
towards misguided motor axons (red arrow in G). (H,I)
trIs10; sem-5(RNAi) worms show numerous EMEs (yellow arrow in H) but
commissural axon guidance is normal (red arrow in I). Scale bar: 50 µm.
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