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Fig. 5. Hair cells are produced early and in excess in the Delta1
conditional knockout cochlea. Confocal optical sections of whole mounts of
cochleas at E17.5. (A) In the apex of the wild-type mouse cochlea,
stained with phalloidin, no hair cells are visible as yet. (B,B') In
the apex of the cochlea of a Delta1 conditional knockout littermate,
hair cells are already plainly visible and present in excess (arrow; B' shows
detail). (C) Middle region of wild-type cochlea shows a narrow band of
Jag1 staining, corresponding to the future sensory patch. (D)
Delta1 conditional knockout shows a wider band of Jag1 staining,
corresponding to the enlarged and precocious sensory patch, and suggesting an
increase in the numbers of supporting cells (which are Jag1-positive) as well
as hair cells (which are not). (E,F) A milder excess of hair
cells is seen in the middle and basal parts of the Delta1 conditional
knockout cochlea (F), with one extra row of outer hair cells and many more
inner hair cells than in the littermate control (E). (G,H)
Confocal optical sections of the middle region of the cochlea in a plane
passing through the bodies of the supporting cells at a deeper level than the
hair cells. The membranes of the supporting cells are stained with Jag1
antibody, and the mutant has slightly more of these cells per unit length of
cochlea.
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