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Fig. 6. Cell-cycle regulation of the rblA promoter. Wild-type cells
bearing the rblA::
pgal reporter were synchronized by
cold shock. Over an 8-hour period, reporter activity was followed using
chemoluminescence, nuclear DNA synthesis was monitored by BrdU pulse-labeling
and cell number measured using a Coulter counter. Gal activities in RLU/µg
protein and BrdU incorporation in percent of total nuclei were normalized to
the respective average over the entire experiment.