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Fig. 2. ichabod maps near the ß-catenin-2 locus in the
telomeric region of LG19. (A) Linkage map of the distal region of
LG19 obtained from a map cross of an ichabod female and
brass male (see text for description). Map positions (in cM) are
obtained from the latest update of the integrated map of the zebrafish genome
(see Day et al. at
http://zfin.org).
Two markers were tested for map position 91.5 cM (z68894/z24777) and for map
position 91.6 (z1799/CA91.6-1) and single markers were tested at other map
positions. Recombination frequencies (recombinants/meiosis tested) between
ichabod and each of the markers are shown below the map. In addition
to testing SSLP markers from the MGH microsatellite map (`Z' markers), we
tested two additional markers: (1) CA85.6-3, a polymorphic CA-repeat marker
located 1.5 kb from the non-polymorphic marker, Z26695, previously mapped to
position 85.6 cM on LG19; and (2) CA(91.6)-1, a polymorphic CA-repeat marker
found within the 3'UTR of ß-catenin-2 cDNA [an EST for
ß-catenin-2, fc16f05/mgc:65770 had previously been mapped to
91.6 on the heat shock meiotic panel by Ian Woods and William Talbot
(unpublished)]. The closest marker showing recombination with ichabod
was CA(85.6)-3, located 1.1 cM away. No recombinants were obtained between
ichabod and z68894, z24777, CA(91.6)-1, z1799, z25291 and z61401,
even though these markers were spread over a region extending almost 10 cM
from CA(85.6)-3. (B) Linkage map of SSLP markers determined from a
completely independent map cross (generated from a WIK x
OregonAB/TübingenWT cross). To test if the map positions in the
non-recombinant region of the map cross shown in A were correct, we determined
recombination frequencies using markers that spanned this region. As is
indicated, in contrast to the ichabod map cross, we did observe
recombination in this region of LG19 (except between markers z14236 and
z25291).
