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Fig. 3. Maternal ß-catenin-2 transcript is reduced in ichabod
embryos. (A) RT-PCR assay of RNA expression levels in wild-type and
ichabod embryos. For each stage shown, oligo dT-primed cDNA was used
as a template for three separate PCR amplifications using primers for
ß-catenin-1, ß-catenin-2 and ef1
.
Maternal ß-catenin-2 transcript was reduced in ichabod embryos,
but reduction of maternal ß-catenin-1 transcript or zygotic transcripts
of either gene was not observed. (B-M) Whole-mount in situ
hybridization showing ubiquitous expression of both ß-catenin genes and
low expression of maternal ß-catenin-2. One-cell stage (top row:
B,E,H,K), sphere stage (middle row: C,F,I,L) and 90% epiboly (bottom row:
D,G,J,M) wild-type (B-D, H-J) and ichabod (E-G, K-M) embryos were
tested for expression of ß-catenin-1 (B-G) and
ß-catenin-2 (H-M). Embryos in D,J are shown in lateral view with
dorsal towards the right.
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