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Fig. 1. Transcriptional activity in the C terminus of Nkx2-5. (A)
Domain structure of the Nkx2-5 C terminus fused to GAL4 DNA-binding domain
(GAL4). (B-E) Trans-activation of the GAL4-dependent G5T-luciferase
reporter in C3H10T1/2 cells by co-transfected GAL-Nkx2-5(232-318) and similar
constructs bearing mutations (see text). Relative transcriptional activity
compared with vector-only control (bars indicate s.e.m.) is shown on the right
of each panel. (F) Western blot showing that GAL-Nkx2-5 proteins are
stably expressed in transfected COS cells.