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Fig. 2. Cephalopod mollusc cardiac NK2 proteins carry the YRD and show a domain
structure resembling their mammalian relatives. (A) Homeodomain and
YRD sequences from cephalopod and other cardiac NK2 proteins. Tyrosine 54
within the homeodomain, a defining hallmark of NK2-class homeodomains, is
shown in red. Tyrosines and phenylalanines within the YRD are shown in red,
with other conserved amino acids in green. Species abbreviations: Ag,
Anopheles gambiae (mosquito); Am, Apis mellifera (honeybee);
amphi, amphioxus; c, chick; Ce, Caenorhabditis elegans (nematode);
Cn, cnidarian Hydra vulgaris (hydra); Dm, Drosophila
melanagaster; h, human; m, mouse; Nv, Nematostella vectensis
(sea anemone); Ok, Octopus kaurna (octopus); So, Sepia
officinalis (cuttlefish); x, Xenopus laevis (frog); z,
zebrafish. TN, Tin-domain. (B-D) LpNkx2-5 expression in Loligo
paelii (squid) embryos. (B) Schematic diagram of a squid embryo
showing location of systemic heart (sh; red) and branchial hearts (bh;
yellow). (C) Histological section showing systemic heart and ink sac
(is). (D) In situ hybridisation to an adjacent section showing
LpNkx2-5 expression in the systemic heart and ink sac (arrow), as
well as muscle beneath the fins (f; arrowheads). (E) Phylogenetic
relationships and domain structure of NK2 protein from divergent species. G,
GIRAW motif;N, Nkx2-5 box; NK2, NK2SD.
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