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Fig. 6. High-level Nkx2-5^GFP/lacZ or Nkx2-5^GFP/Y-A ES cell contribution to chimaeric embryos perturbs heart development. (A,B) Nkx2-5^GFP/Y-A ${leftrightarrow}$ wild-type chimaeras at 7.5 and 8.5 dpc stained for lacZ. (C) Chimaera showing abnormal cardiac development at 9.0 dpc with pericardial oedema (arrows). (D-I) Comparison of heart morphology (upper panels) and histology (lower panels) in 9.0 dpc chimaeric embryos composed of indicated ES cell genotypes. Nkx2-5^lacZ/+ ${leftrightarrow}$ wild-type chimaeras (D,G) were morphologically normal. Nkx2-5^GFP/lacZ ${leftrightarrow}$ wild-type (E,H) and Nkx2-5^GFP/Y-A ${leftrightarrow}$ wild-type (F,I) chimaeras showed arrested heart development and absence of ventricular trabeculation (arrows). (J,K) High-level chimaera at E9.5 carrying Nkx2-5^{Y-A:IRESlacZ/+} ES cells (stained for lacZ) and section, showing relatively normal cardiac morphology. Abbreviations: CA, common atrium; LV, left ventricle; OT, outflow tract; PV, primitive ventricle; RA, right atrium.

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