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Fig. 5. Sox9 and Snail2 form a protein complex. (A) Schematic diagram of wild type (wt) and deletion mutants ( ${Delta}$ C and ${Delta}$ N) of HA-tagged Sox9 and GST-tagged Snail2. (B) Interaction of GST-Snail2 deletions and full-length HA-Sox9. Extract of HA-Sox9-transfected COS7 cells were incubated with full-length (wt) and deletions of GST-Snail2 ( ${Delta}$ N and ${Delta}$ C), and immunoprecipitated with anti-HA beads. Full-length and ${Delta}$ N-Snail2 is detected in precipitated fractions by anti-GST. (C) Interaction of full-length GST-Snail2 and deletions of HA-Sox9. Extracts of COS7 cells transfected with HA-tagged full-length (wt) Sox9 or deletions ( ${Delta}$ N and ${Delta}$ C) were incubated with GST-Snail2 ( ${Delta}$ N) and immunoprecipitated with anti-HA beads. With anti-GST, precipitation of Snail2 is detected when incubated with extracts containing either full-length Sox9 (wt) or ${Delta}$ C-Sox9.

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